Illumina HiSeq 3000/HiSeq 4000 Sequencing Platforms Systems

HiSeq 3000 System	HiSeq 4000 System
No. of Flow Cells per Run	1	1 or 2
Data Yield - 2 × 150 bp	650–750 Gb	1300–1500 Gb
Data Yield - 2 × 75 bp	325–375 Gb	650–750 Gb
Data Yield - 1 × 50 bp	105–125 Gb	210–250 Gb
Clusters Passing Filter (8 lanes per flow cell)	up to 2.5B single reads or 5B paired end reads	up to 5B single reads or 10B PE reads
Quality Scores - 2 × 50 bp	≥ 85% bases above Q30	≥ 85% bases above Q30
Quality Scores - 2 × 75 bp	≥ 80% bases above Q30	≥ 80% bases above Q30
Quality Scores - 2 × 150 bp	≥ 75% bases above Q30	≥ 75% bases above Q30
Daily Throughput	> 200 Gb	> 400 Gb
Run Time	< 1–3.5 days	< 1–3.5 days
Human Genomes per Run*	up to 6	up to 12
Exomes per Run†	up to 48	up to 96
Transcriptomes per Run‡	up to 50	up to 100

List number: - R

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